anti γ2 (clone cl2980; atlas ) (Atlas Antibodies)

Atlas Antibodies anti γ2 (clone cl2980; atlas )
Anti γ2 (Clone Cl2980; Atlas ), supplied by Atlas Antibodies, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti γ2 (clone cl2980; atlas )/product/Atlas Antibodies
Average 93 stars, based on 1 article reviews

anti γ2 (clone cl2980; atlas ) - by Bioz Stars, 2026-03

93/100 stars

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laminin (Boster Bio)

Boster Bio laminin

Laminin, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/laminin/product/Boster Bio
Average 93 stars, based on 1 article reviews

laminin - by Bioz Stars, 2026-03

93/100 stars

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mouse anti-lamb2/laminin b2 monoclonal antibody (Becton Dickinson)

Becton Dickinson mouse anti-lamb2/laminin b2 monoclonal antibody

Autophagy promotes tubule-like structure formation by GSCs in 3D scaffold. (A) GSCs transfected with EGFP-LC3 were cultured in DMEM, STM or EBM for 72 h. Confocal microscopy was used to assess autophagy shown as green spots (left panel). Two hundred cells were randomly chosen to count the number of EGFP-LC3 fluorescent spots (right panel). Scale bar: 10 μm. (B) Confocal microscopy showing EGFP-transfected GSCs to form tubule-like structure in 3D scaffold (left panel). The number of tubular structures formed by GSCs in 3D scaffold was analyzed (right pannel). Scale bar: 20 μm. Asterisks (*) indicate VM channel formed by tumor cells. (C) Expression of autophagy proteins, ATG5, ATG7, LC3-I to LC3-II conversion and SQSTM1. TUBB/β-Tubulin served as a control. (D) The expressions of PROM1, GFAP, KDR proteins and the phosphorylation of Y1175 in the C-terminal region of KDR (p-KDR) as well as CDH5 and <t>LAMB2.</t> (E) Double staining of LC3 and CDH5 as well as LC3 and LAMB2 in GSCs cultured with EBM on the scaffold. LC3 is marked as red spots in the cytoplasm; CDH5 and LAMB2 are in green color in the cytoplasm. Scale bar: 20 μm. Nuclei were stained by Hoechst 33324 in blue. The results are expressed as the mean ± SEM from 3 experiments.

Mouse Anti Lamb2/Laminin B2 Monoclonal Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti-lamb2/laminin b2 monoclonal antibody/product/Becton Dickinson
Average 90 stars, based on 1 article reviews

mouse anti-lamb2/laminin b2 monoclonal antibody - by Bioz Stars, 2026-03

90/100 stars

Buy from Supplier

Image Search Results

Journal: International Journal of Molecular Sciences

Article Title: The Role of Sensory Nerves in Dental Pulp Homeostasis: Histological Changes and Cellular Consequences after Sensory Denervation

doi: 10.3390/ijms25021126

Figure Lengend Snippet: Antibodies used in this study.

Article Snippet: Laminin , Rabbit , Boster , PA1581.

Techniques:

Journal: Autophagy

Article Title: Autophagy-induced KDR/VEGFR-2 activation promotes the formation of vasculogenic mimicry by glioma stem cells

doi: 10.1080/15548627.2017.1336277

Figure Lengend Snippet: Autophagy promotes tubule-like structure formation by GSCs in 3D scaffold. (A) GSCs transfected with EGFP-LC3 were cultured in DMEM, STM or EBM for 72 h. Confocal microscopy was used to assess autophagy shown as green spots (left panel). Two hundred cells were randomly chosen to count the number of EGFP-LC3 fluorescent spots (right panel). Scale bar: 10 μm. (B) Confocal microscopy showing EGFP-transfected GSCs to form tubule-like structure in 3D scaffold (left panel). The number of tubular structures formed by GSCs in 3D scaffold was analyzed (right pannel). Scale bar: 20 μm. Asterisks (*) indicate VM channel formed by tumor cells. (C) Expression of autophagy proteins, ATG5, ATG7, LC3-I to LC3-II conversion and SQSTM1. TUBB/β-Tubulin served as a control. (D) The expressions of PROM1, GFAP, KDR proteins and the phosphorylation of Y1175 in the C-terminal region of KDR (p-KDR) as well as CDH5 and LAMB2. (E) Double staining of LC3 and CDH5 as well as LC3 and LAMB2 in GSCs cultured with EBM on the scaffold. LC3 is marked as red spots in the cytoplasm; CDH5 and LAMB2 are in green color in the cytoplasm. Scale bar: 20 μm. Nuclei were stained by Hoechst 33324 in blue. The results are expressed as the mean ± SEM from 3 experiments.

Article Snippet: Antibodies used include mouse anti-LAMB2/laminin B2 monoclonal antibody (mAb; BD Biosciences, 610722), rabbit anti-CDH5/cadherin 5 mAb (Cell Signaling Technology, 2158), rabbit anti-PROM1/CD133 polyclonal antibody (pAb; Santa Cruz Biotechnology, sc-30220), rabbit anti-GFAP pAb (Abcam, ab7260), mouse anti-CD34 mAb (Abcam, ab187282), rabbit anti-VEGF pAb (Proteintech, 19003–1-AP), rabbit anti-phospho- KDR/VEGFR-2 mAb (Cell Signaling Technology, 2478), rabbit anti-KDR/VEGFR-2 mAb (Cell Signaling Technology, 2479), mouse anti-LC3B mAb (Santa Cruz Biotechnology, sc-376404), rabbit anti-ATG5 mAb (Cell Signaling Technology, 8540), rabbit anti-ATG7 mAb (Cell Signaling Technology, 8558), rabbit anti-SQSTM1/p62 pAb (Sigma-Aldrich, P0067), rabbit anti-BECN1 mAb (Abcam, ab51031), mouse anti-PI3K p85 mAb (Abcam, ab86714), rabbit anti-pan-AKT pAb (Abcam, ab8805), rabbit anti-phospho-PI3K p85 (Y607) pAb (Abcam, ab182651), rabbit anti-phospho-pan-AKT pAb (Abcam, ab38449).

Techniques: Transfection, Cell Culture, Confocal Microscopy, Expressing, Double Staining, Staining

Tubular structure formation by GSCs is promoted by rapamycin (RAPA). (A) RAPA (100 nM) was added to EBM and confocal microscopy was used to detect autophagic vesicles in GSCs forming tubular structure (left panel). Two hundred cells were randomly chosen to count green fluorescent spots within the cells (right panel). Scale bar: 10 μm. (B) Confocal microscopy showing EGFP-transfected GSCs to form tubule-like structure in 3D scaffold (left panel). The number of tubular structures was counted in the scaffold cultured with or without RAPA (right panel). Scale bar: 20 μm. Asterisks (*) indicate VM channel formed by tumor cells. (C) The expression of LC3-I to LC3-II conversion, SQSTM1 and VM-related proteins (KDR, p-KDR, CDH5 and LAMB2) in GSCs treated by RAPA. The results are expressed as the mean ± SEM from 3 experiments.

Journal: Autophagy

Article Title: Autophagy-induced KDR/VEGFR-2 activation promotes the formation of vasculogenic mimicry by glioma stem cells

doi: 10.1080/15548627.2017.1336277

Figure Lengend Snippet: Tubular structure formation by GSCs is promoted by rapamycin (RAPA). (A) RAPA (100 nM) was added to EBM and confocal microscopy was used to detect autophagic vesicles in GSCs forming tubular structure (left panel). Two hundred cells were randomly chosen to count green fluorescent spots within the cells (right panel). Scale bar: 10 μm. (B) Confocal microscopy showing EGFP-transfected GSCs to form tubule-like structure in 3D scaffold (left panel). The number of tubular structures was counted in the scaffold cultured with or without RAPA (right panel). Scale bar: 20 μm. Asterisks (*) indicate VM channel formed by tumor cells. (C) The expression of LC3-I to LC3-II conversion, SQSTM1 and VM-related proteins (KDR, p-KDR, CDH5 and LAMB2) in GSCs treated by RAPA. The results are expressed as the mean ± SEM from 3 experiments.

Techniques: Confocal Microscopy, Transfection, Cell Culture, Expressing

Autophagy mediates the formation of VM through KDR phosphorylation. (A) Confocal microscopy detection of autophagic vesicles formed by GSCs transfected with EGFP-LC3 and treated with 5 μM chloroquine (CQ) (left panel). Two hundred cells were randomly chosen to count green fluorescent spots within the cells (right panel). Scale bar: 10 μm. (B) Confocal microscopy showing EGFP-transfected GSCs to form vascular tubules in 3D scaffold (left panel). The number of tubular structures was counted in the scaffold with or without CQ (right panel). Scale bar: 20 μm. Asterisks (*) indicate VM channels formed by tumor cells. (C) The expression of LC3-I to LC3-II conversion, SQSTM1 and KDR, p-KDR, LAMB2, and CDH5 in GSCs after 5 μM CQ treatment. (D) Western blot detection of p-KDR, KDR, LAMB2, CDH5 and SQSTM1 in GSCs in medium with 100 μg/ml BEV and/or 5 μM CQ. The results are expressed as the mean ± SEM from 3 experiments.

Journal: Autophagy

Article Title: Autophagy-induced KDR/VEGFR-2 activation promotes the formation of vasculogenic mimicry by glioma stem cells

doi: 10.1080/15548627.2017.1336277

Figure Lengend Snippet: Autophagy mediates the formation of VM through KDR phosphorylation. (A) Confocal microscopy detection of autophagic vesicles formed by GSCs transfected with EGFP-LC3 and treated with 5 μM chloroquine (CQ) (left panel). Two hundred cells were randomly chosen to count green fluorescent spots within the cells (right panel). Scale bar: 10 μm. (B) Confocal microscopy showing EGFP-transfected GSCs to form vascular tubules in 3D scaffold (left panel). The number of tubular structures was counted in the scaffold with or without CQ (right panel). Scale bar: 20 μm. Asterisks (*) indicate VM channels formed by tumor cells. (C) The expression of LC3-I to LC3-II conversion, SQSTM1 and KDR, p-KDR, LAMB2, and CDH5 in GSCs after 5 μM CQ treatment. (D) Western blot detection of p-KDR, KDR, LAMB2, CDH5 and SQSTM1 in GSCs in medium with 100 μg/ml BEV and/or 5 μM CQ. The results are expressed as the mean ± SEM from 3 experiments.

Techniques: Confocal Microscopy, Transfection, Expressing, Western Blot

Knockdown of ATG5 reduces tubular structure formation by GSCs and phosphorylation of KDR. (A) The expression of ATG5, p-KDR, KDR, CDH5 and LAMB2 in GSCs transfected with siATG5. (B) Two hundred cells were randomly chosen to count green fluorescent spots within the cells. (C) The number of tubular structures formed by GSCs and siATG5-GSCs in the scaffold counted with confocal microscopy. (D) Western blot detection of p-KDR and KDR in siATG5-GSCs cultured with 100 μg/ml BEV. (E) and (F) Mice with intracranial injection of GSCs transfected with siCtrl RNA or siATG5 were treated with BEV. Forty-two animals were then randomly divided into 7 groups consisting of 6 animals each. After 4 wk, tumors were collected to analyze vessel density (E) and formation of VM (F). HPF, high-power field. The results are expressed as the mean ± SEM from 3 experiments.

Journal: Autophagy

Article Title: Autophagy-induced KDR/VEGFR-2 activation promotes the formation of vasculogenic mimicry by glioma stem cells

doi: 10.1080/15548627.2017.1336277

Figure Lengend Snippet: Knockdown of ATG5 reduces tubular structure formation by GSCs and phosphorylation of KDR. (A) The expression of ATG5, p-KDR, KDR, CDH5 and LAMB2 in GSCs transfected with siATG5. (B) Two hundred cells were randomly chosen to count green fluorescent spots within the cells. (C) The number of tubular structures formed by GSCs and siATG5-GSCs in the scaffold counted with confocal microscopy. (D) Western blot detection of p-KDR and KDR in siATG5-GSCs cultured with 100 μg/ml BEV. (E) and (F) Mice with intracranial injection of GSCs transfected with siCtrl RNA or siATG5 were treated with BEV. Forty-two animals were then randomly divided into 7 groups consisting of 6 animals each. After 4 wk, tumors were collected to analyze vessel density (E) and formation of VM (F). HPF, high-power field. The results are expressed as the mean ± SEM from 3 experiments.

Techniques: Expressing, Transfection, Confocal Microscopy, Western Blot, Cell Culture, Injection

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